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Testing

How a peptide
earns the lot number.

Buying research peptides comes down to one question: is what's in the vial what the label says it is? Every batch we sell is tested by Freedom Diagnostics Testing, an independent US-based analytical lab. Here's how the process actually works.

01

We ship a sample from every batch to Freedom Diagnostics.

When a new batch of peptide comes in, we pull a representative sample, label it with the lot number, and ship it to Freedom Diagnostics Testing in Franklin, Tennessee. Freedom Diagnostics is an independent US-based analytical lab — we don't own it, and we don't get to influence the result.

02

They test it by HPLC, mass spec, and LAL.

Freedom Diagnostics runs the sample through high-performance liquid chromatography (HPLC) to measure purity by mass percentage, and mass spectrometry to confirm the molecular identity of the peptide. They also run an LAL (Limulus Amebocyte Lysate) assay to measure bacterial endotoxin — reported in EU/mL, with a pass threshold of ≤ 0.05 EU/mL. These are the same methods pharmaceutical labs and academic researchers use to verify reagents.

03

Freedom Diagnostics issues the Certificate of Analysis.

The COA is the lab's signed report — what was tested, what methods were used, and what the results were. It carries Freedom Diagnostics' name and accession number, not ours; we can't edit it. Every COA they issue is also added to their public archive, so anyone can independently look it up by accession number.

04

We publish the COA on the product page.

The COA for each product is posted directly on that product's page — open it before you buy, no sign-up, no "contact us for the report." If a batch ever fails to meet our purity threshold, that batch doesn't get listed. You can also verify any COA at freedomdiagnosticstesting.com using the accession number printed on the report.

Why third-party and not in-house?
An in-house test from the supplier is a self-graded paper. The point of independent testing is that the result isn't ours to influence. If Freedom Diagnostics finds something wrong, that's the result we publish.
Can I verify the COA myself?
Yes. Every Freedom Diagnostics COA carries an accession number. Their public archive at freedomdiagnosticstesting.com lets anyone search by accession number, search code, or company name and pull up the original PDF. If the report on our site doesn't match the one in their archive, you'd see it immediately.
What's the difference between identity testing and purity testing?
Identity testing (mass spectrometry) confirms the molecule in the vial actually is the peptide on the label. Purity testing (HPLC) measures how much of the contents is that peptide versus byproducts, residual reagents, or degradation. A real verification needs both.
Why does endotoxin testing matter?
Endotoxin is a fragment of the outer membrane of gram-negative bacteria. It can be present even in a peptide that's chemically pure — it comes from the water, glassware, or handling during manufacture, not the peptide itself. At nanogram levels it triggers inflammatory responses in cell culture and in vivo work, which is why a peptide can read 99% pure by HPLC and still wreck an experiment. Freedom Diagnostics measures it by LAL assay and reports a numeric result in EU/mL — every batch we sell tests at or below 0.05 EU/mL.
What happens if a batch fails?
It doesn't get listed for sale. We either return it to the manufacturer or destroy it. The failed COA is kept on file either way.

Every product page carries its own report.

Browse the catalogueQuestions about a lot? →